1EX7
CRYSTAL STRUCTURE OF YEAST GUANYLATE KINASE IN COMPLEX WITH GUANOSINE-5'-MONOPHOSPHATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-12-05 |
| Detector | MAC Science DIP-2000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 50.625, 50.625, 155.327 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.900 |
| R-factor | 0.156 * |
| Rwork | 0.170 |
| R-free | 0.25700 |
| Structure solution method | REFINEMENT OF THE MODEL |
| Starting model (for MR) | 1gky |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.033 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.095 | 0.378 |
| Total number of observations | 72765 * | |
| Number of reflections | 15836 | |
| <I/σ(I)> | 14.6 | 1.8455 |
| Completeness [%] | 94.4 | 86.9 |
| Redundancy | 4.6 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 292 | drop consists of equal amounts of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | drop | GMP | 10 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
| 5 | 1 | reservoir | sodium phosphate | 50 (mM) |
