1EX4
HIV-1 INTEGRASE CATALYTIC CORE AND C-TERMINAL DOMAIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-06-08 |
| Detector | MARRESEARCH |
| Spacegroup name | P 3 1 2 |
| Unit cell lengths | 103.990, 103.990, 101.380 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.260 - 2.800 |
| R-factor | 0.258 |
| Rwork | 0.258 |
| R-free | 0.30600 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 22.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.077 | 0.840 |
| Total number of observations | 178921 * | |
| Number of reflections | 15580 | |
| <I/σ(I)> | 22 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 11.5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | hepes, NaCL, CHAPS, formate, citrate, DTT, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Na formate | 2.2 (M) | |
| 2 | 1 | reservoir | Na citrate | 150 (mM) | |
| 3 | 1 | reservoir | dithiothreitol | 3 (mM) | |
| 4 | 1 | reservoir | CHAPS | 3 (mM) |
