1EVS
CRYSTAL STRUCTURE OF HUMAN ONCOSTATIN M
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-11-22 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.887, 53.285, 106.694 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.200 |
R-factor | 0.205 * |
Rwork | 0.205 |
R-free | 0.26100 |
RMSD bond length | 0.015 |
RMSD bond angle | 19.888 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.250 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.083 | 0.376 |
Total number of observations | 43901 * | |
Number of reflections | 10513 | |
<I/σ(I)> | 13.2 | |
Completeness [%] | 96.5 | 98.2 |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | PEG 35000, ammonium acetate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 22K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 50 (mM) | |
3 | 1 | drop | 150 (mM) | ||
4 | 1 | reservoir | PEG35000 | 30 (%(w/v)) | |
5 | 1 | reservoir | sodium acetate | 0.25 (M) |