1EVS

CRYSTAL STRUCTURE OF HUMAN ONCOSTATIN M

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-11-22
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	35.887, 53.285, 106.694
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.200
R-factor	0.205 *
Rwork	0.205
R-free	0.26100
RMSD bond length	0.015
RMSD bond angle	19.888 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.250
High resolution limit [Å]	2.200	2.200
Rmerge	0.083	0.376
Total number of observations	43901 *
Number of reflections	10513
<I/σ(I)>	13.2
Completeness [%]	96.5	98.2
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	PEG 35000, ammonium acetate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 22K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)
3	1	drop		150 (mM)
4	1	reservoir	PEG35000	30 (%(w/v))
5	1	reservoir	sodium acetate	0.25 (M)