1EVG
CRYSTAL STRUCTURE ANALYSIS OF CYS167 MUTANT OF ESCHERICHIA COLI WITH UNMODIFIED CATALYTIC CYSTEINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-11-25 |
| Detector | RIGAKU RAXIS |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 131.973, 131.973, 131.973 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.000 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.22500 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 24.000 * |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 6.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.083 | 0.214 |
| Number of reflections | 24936 | |
| <I/σ(I)> | 25.79 | |
| Completeness [%] | 100.0 | 99.6 |
| Redundancy | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 291 | 48% saturated ammonium sulfate, 100 mM Tris HCl, 20 mM 2-mercaptoethanol , pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 18K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 7-10 (mg/ml) | |
| 2 | 1 | drop | EDTA | 1 (mM) | |
| 3 | 1 | drop | 2-mercaptoethanol | 15 (mM) | |
| 4 | 1 | drop | potassium chloride | 50-100 (mM) | |
| 5 | 1 | drop | potassium phosphate | 20-50 (mM) | |
| 6 | 1 | reservoir | ammonium sulfate | 48 (%sat) | |
| 7 | 1 | reservoir | 2-mercaptoethanol | 20 (mM) | |
| 8 | 1 | reservoir | Tris-HCl | 100 (mM) |
