1EV9
RAT GLUTATHIONE S-TRANSFERASE A1-1 MUTANT W21F WITH GSO3 BOUND
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 1997-09-29 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 84.700, 275.100, 70.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.800 * - 2.200 |
R-factor | 0.236 * |
Rwork | 0.236 |
R-free | 0.29900 |
RMSD bond length | 0.013 |
RMSD bond angle | 2.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.843) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.880 |
High resolution limit [Å] | 1.980 * | 1.980 * |
Rmerge | 0.085 * | 0.670 * |
Number of reflections | 57919 * | 8678 * |
<I/σ(I)> | 13.6 | |
Completeness [%] | 97.0 * | 88 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 298 | 0.2M lithium sulfate, 50% saturated ammonium sulfate, 0.1M 3-cyclohexylamino-1-propane sulfonic acid (CAPS), pH 10.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | 3-[cyclohexylaminoo]-1-propane sulfonic acid | 0.1 (M) | |
3 | 1 | drop | 0.2 (M) | ||
4 | 1 | drop | ammonium sulfate | 50 (%sat) |