1EV2

CRYSTAL STRUCTURE OF FGF2 IN COMPLEX WITH THE EXTRACELLULAR LIGAND BINDING DOMAIN OF FGF RECEPTOR 2 (FGFR2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	110
Detector technology	AREA DETECTOR
Collection date	1999-09-22
Detector	SDMS
Spacegroup name	P 1
Unit cell lengths	72.198, 71.677, 90.920
Unit cell angles	90.53, 89.98, 89.99

Refinement procedure

Resolution	25.000 - 2.200
R-factor	0.248
Rwork	0.248
R-free	0.27300
RMSD bond length	0.007
RMSD bond angle	0.782 *
Data reduction software	SDMS
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.042	0.241
Total number of observations	206913 *
Number of reflections	93440 *
<I/σ(I)>	16.3
Completeness [%]	96.3	87.8
Redundancy	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5	298	drop consists of equal volume of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris-HCl	25 (mM)
3	1	drop		150 (mM)
4	1	reservoir	PEG4000	10-15 (%)
5	1	reservoir	isopropanol	10 (%)
6	1	reservoir	HEPES-NaOH	0.1 (M)