1EUU

SIALIDASE OR NEURAMINIDASE, LARGE 68KD FORM

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Detector technology	IMAGE PLATE
Collection date	1995-05
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	50.870, 116.990, 60.000
Unit cell angles	90.00, 95.60, 90.00

Refinement procedure

Resolution	6.000 - 2.500
R-factor	0.228
Rwork	0.228
RMSD bond length	0.012
RMSD bond angle	1.960
Data reduction software	DENZO
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.500 *	2.500 *
Rmerge	0.054	0.118 *
Total number of observations	35106 *
Number of reflections	16297
Completeness [%]	59.3	49.3 *
Redundancy	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *			Taylor, G., (1992) J.Mol.Biol., 225, 1135. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3350	8 (%)