1ETH
TRIACYLGLYCEROL LIPASE/COLIPASE COMPLEX
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Detector technology | IMAGE PLATE |
| Spacegroup name | F 2 3 |
| Unit cell lengths | 289.100, 289.100, 289.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.800 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.29000 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 27.220 * |
| Data reduction software | MOSFLM |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 12.400 |
| High resolution limit [Å] | 2.800 |
| Rmerge | 0.130 |
| Number of reflections | 42501 |
| Completeness [%] | 86.0 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6 * | 20 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | lipase | 10 (mg/ml) | 0.01ml |
| 2 | 1 | drop | colipase | 10 (mg/ml) | 0.007 ml |
| 3 | 1 | reservoir | MES | 50 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 0.5 (M) | |
| 5 | 1 | reservoir | TGME | 15 (mM) |
