1ETE
CRYSTAL STRUCTURE OF THE FLT3 LIGAND
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1996-01-25 |
| Detector | PRINCETON 2K |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 137.080, 159.410, 26.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| Rwork | 0.239 |
| R-free | 0.28700 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.062 | 0.221 |
| Number of reflections | 29421 | |
| <I/σ(I)> | 12 | |
| Completeness [%] | 96.9 | 95.5 |
| Redundancy | 4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.4 | 298 | drop consists of 1:1 mixture of well and protein solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-15 (mg/ml) | |
| 2 | 1 | drop | HEPES | 20 (mM) | |
| 3 | 1 | drop | EDTA | 1 (mM ) | |
| 4 | 1 | reservoir | PEG8000 | 6-9 (%(w/v)) | |
| 5 | 1 | reservoir | zinc acetate | 0.2 (M) | |
| 6 | 1 | reservoir | sodium cacodylate | 0.1 (M) |
