1ETE

CRYSTAL STRUCTURE OF THE FLT3 LIGAND

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	1996-01-25
Detector	PRINCETON 2K
Spacegroup name	P 21 21 2
Unit cell lengths	137.080, 159.410, 26.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.200
Rwork	0.239
R-free	0.28700
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.062	0.221
Number of reflections	29421
<I/σ(I)>	12
Completeness [%]	96.9	95.5
Redundancy	4	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.4	298	drop consists of 1:1 mixture of well and protein solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-15 (mg/ml)
2	1	drop	HEPES	20 (mM)
3	1	drop	EDTA	1 (mM )
4	1	reservoir	PEG8000	6-9 (%(w/v))
5	1	reservoir	zinc acetate	0.2 (M)
6	1	reservoir	sodium cacodylate	0.1 (M)