1ES0

CRYSTAL STRUCTURE OF THE MURINE CLASS II ALLELE I-A(G7) COMPLEXED WITH THE GLUTAMIC ACID DECARBOXYLASE (GAD65) PEPTIDE 207-220

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	96
Detector technology	IMAGE PLATE
Collection date	1999-01-16
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	95.149, 110.123, 96.084
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.960 - 2.600
R-factor	0.21
Rwork	0.210
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	I-A(K) - PDB CODE 1IAK
RMSD bond length	0.006
RMSD bond angle	26.000 *
Data reduction software	DENZO
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.960	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.068 *	0.353 *
Total number of observations	66107 *
Number of reflections	15754
<I/σ(I)>	15.7	4.2
Completeness [%]	99.3	99.6
Redundancy	4.2	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.5 *	277	seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	10 (mg/ml)
2	1	1	HEPES	20 (mM)
3	1	1		25 (mM)