1EQB
X-RAY CRYSTAL STRUCTURE AT 2.7 ANGSTROMS RESOLUTION OF TERNARY COMPLEX BETWEEN THE Y65F MUTANT OF E-COLI SERINE HYDROXYMETHYLTRANSFERASE, GLYCINE AND 5-FORMYL TETRAHYDROFOLATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-03-26 |
| Detector | RIGAKU |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.740, 172.480, 95.410 |
| Unit cell angles | 90.00, 104.28, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.23600 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 22.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.280 | 1.000 |
| Number of reflections | 63328 | |
| <I/σ(I)> | 5.53 | |
| Completeness [%] | 94.8 | 96.1 * |
| Redundancy | 5.1 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.2 | 298 | 2M K3PO4, pH 7.2, VAPOR DIFFUSION, temperature 298K |
