1EO3
INHIBITION OF ECORV ENDONUCLEASE BY DEOXYRIBO-3'-S-PHOSPHOROTHIOLATES: A HIGH RESOLUTION X-RAY CRYSTALLOGRAPHIC STUDY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-07-12 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.000, 48.500, 63.800 |
| Unit cell angles | 97.00, 108.90, 106.30 |
Refinement procedure
| Resolution | 4.900 - 2.000 |
| R-factor | 0.196 * |
| Rwork | 0.196 |
| R-free | 0.29000 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.120 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.131 | 0.358 |
| Total number of observations | 55892 * | |
| Number of reflections | 31662 | |
| <I/σ(I)> | 7.4 | |
| Completeness [%] | 92.8 | 90.5 |
| Redundancy | 1.77 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 | 17 * | 25% PEG 4000, 0.1M HEPES, 0.15M NaCl, 50 mM MgCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | HEPES | ||
| 2 | 1 | 1 | NaCl | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 1 | PEG 4000 | ||
| 5 | 1 | 2 | PEG 4000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | EcoRV | 10 (mg/ml) | |
| 2 | 1 | drop | DNA | 1.0 (mg/ml) | |
| 3 | 1 | reservoir | HEPES | 100 (mM) | |
| 4 | 1 | reservoir | 150 (mM) | ||
| 5 | 1 | reservoir | PEG4000 | 10-15 (%) | |
| 6 | 1 | reservoir | 50 (mM) |
