1EMH
CRYSTAL STRUCTURE OF HUMAN URACIL-DNA GLYCOSYLASE BOUND TO UNCLEAVED SUBSTRATE-CONTAINING DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-D |
| Synchrotron site | APS |
| Beamline | 14-BM-D |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 1999-08-16 |
| Detector | ADSC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.655, 64.815, 94.964 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.216 * |
| Rwork | 0.216 |
| R-free | 0.23300 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.052 | 0.231 |
| Total number of observations | 116160 * | |
| Number of reflections | 26594 | |
| <I/σ(I)> | 14.9 | |
| Completeness [%] | 93.0 | 76.9 * |
| Redundancy | 4.3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.5 | 297 | PEG 4000, HEPES buffer, NaCl, dioxane, DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | HEPES buffer | ||
| 2 | 1 | 1 | NaCl | ||
| 3 | 1 | 1 | dioxane | ||
| 4 | 1 | 1 | DTT | ||
| 5 | 1 | 1 | PEG 4000 | ||
| 6 | 1 | 2 | PEG 4000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 20 (%) | |
| 2 | 1 | reservoir | HEPES | 100 (mM) | |
| 3 | 1 | reservoir | 10 (%) | ||
| 4 | 1 | reservoir | dithiothreitol | 1 (mM) |
