1EK5
STRUCTURE OF HUMAN UDP-GALACTOSE 4-EPIMERASE IN COMPLEX WITH NAD+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 273 |
Detector technology | AREA DETECTOR |
Collection date | 1999-05-27 |
Detector | SIEMENS HI-STAR |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 50.300, 116.400, 130.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.800 |
R-factor | 0.191 |
Rwork | 0.190 |
R-free | 0.22300 |
RMSD bond length | 0.011 |
RMSD bond angle | 2.360 |
Data scaling software | SAINT |
Phasing software | TNT |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.880 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.059 | 0.258 |
Number of reflections | 34184 | 3761 * |
<I/σ(I)> | 16.5 | |
Completeness [%] | 95.2 | 87 |
Redundancy | 2.9 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG-8000, KCl, HEPPS, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 18-20 (%) | |
2 | 1 | reservoir | HEPPS | 100 (mM) | pH7.5 |
3 | 1 | reservoir | 250 (mM) | ||
4 | 1 | drop | protein | 17 (mg/ml) | |
5 | 1 | drop | NAD+ | 2 (mM) |