1EK0
GPPNHP-BOUND YPT51 AT 1.48 A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-06-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 |
| Unit cell lengths | 62.326, 62.326, 91.221 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.000 - 1.480 |
| R-factor | 0.178 |
| Rwork | 0.178 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5p21 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.040 |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.550 |
| High resolution limit [Å] | 1.480 | 1.470 |
| Rmerge | 0.026 | 0.214 |
| Total number of observations | 146199 * | |
| Number of reflections | 33345 | |
| <I/σ(I)> | 27.55 | 5.22 |
| Completeness [%] | 93.9 | 90 |
| Redundancy | 4.4 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 18 * | 10% PEG-3350, 100 MM HEPES, PH 7.5, 20 MM NICL2 , VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 13 (mg/ml) | |
| 2 | 1 | drop | HEPES-NaOH | 25 (mM) | |
| 3 | 1 | drop | 5 (mM) | ||
| 4 | 1 | drop | 50 (mM) | ||
| 5 | 1 | drop | beta-mercaptoethanol | 2 (mM) | |
| 6 | 1 | reservoir | PEG3350 | 10 (%(v/v)) | |
| 7 | 1 | reservoir | HEPES-NaOH | 100 (mM) | |
| 8 | 1 | reservoir | 20 (mM) |
