1EJH
EIF4E/EIF4G PEPTIDE/7-METHYL-GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-10-01 |
| Detector | ADSC QUANTUM 1 |
| Spacegroup name | P 31 |
| Unit cell lengths | 140.600, 140.600, 39.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| Rwork | 0.218 |
| R-free | 0.26100 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.290 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.093 | 0.206 |
| Completeness [%] | 98.9 | 95.8 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | (NH4)2SO4 and Peg400, pH 8.5, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 2.0-2.1 (M) | |
| 2 | 1 | reservoir | PEG400 | 5 (%) | |
| 3 | 1 | reservoir | EDTA | 10 (mM) | |
| 4 | 1 | reservoir | Tris-HCl | 0.1 (M) |
