1EJB

LUMAZINE SYNTHASE FROM SACCHAROMYCES CEREVISIAE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	105
Detector technology	IMAGE PLATE
Collection date	1998-08-25
Detector	MARRESEARCH
Spacegroup name	P 41 21 2
Unit cell lengths	82.893, 82.893, 298.842
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.850
R-factor	0.197
Rwork	0.197
R-free	0.22500
RMSD bond length	0.017
RMSD bond angle	2.470
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *	1.870
High resolution limit [Å]	1.850	1.850
Rmerge	0.044	0.260
Total number of observations	510352 *
Number of reflections	89967
<I/σ(I)>	32
Completeness [%]	99.7	99.6
Redundancy	15.71

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	22 *	sodium phosphate, potassium phosphate, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	14 (mg/ml)
2	1	drop	sodium potassium phosphate	1 (M)
3	1	reservoir	sodium potassium phosphate	1.9 (M)