1EJB
LUMAZINE SYNTHASE FROM SACCHAROMYCES CEREVISIAE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 105 |
Detector technology | IMAGE PLATE |
Collection date | 1998-08-25 |
Detector | MARRESEARCH |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 82.893, 82.893, 298.842 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.850 |
R-factor | 0.197 |
Rwork | 0.197 |
R-free | 0.22500 |
RMSD bond length | 0.017 |
RMSD bond angle | 2.470 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 9999.000 * | 1.870 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.044 | 0.260 |
Total number of observations | 510352 * | |
Number of reflections | 89967 | |
<I/σ(I)> | 32 | |
Completeness [%] | 99.7 | 99.6 |
Redundancy | 15.71 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 22 * | sodium phosphate, potassium phosphate, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 14 (mg/ml) | |
2 | 1 | drop | sodium potassium phosphate | 1 (M) | |
3 | 1 | reservoir | sodium potassium phosphate | 1.9 (M) |