1EJ6
Reovirus core
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 273 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-05-01 |
| Detector | FUJI |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 1255.000, 1255.000, 1255.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.600 |
| R-factor | 0.2081 |
| Rwork | 0.206 |
| R-free | 0.20810 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | CCP4 |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.000 | 3.710 |
| High resolution limit [Å] | 3.600 | 3.470 |
| Rmerge | 0.260 | 0.530 |
| Number of reflections | 928767 | |
| <I/σ(I)> | 2.7 | |
| Completeness [%] | 98.0 | 70 |
| Redundancy | 5.8 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 | 298 | sodium chloride, sodium acetate, magnesium chloride, hepes, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | drop | 0.5 (M) | ||
| 3 | 1 | drop | 0-0.3 (M) | ||
| 4 | 1 | drop | 50 (mM) | ||
| 5 | 1 | drop | HEPES | 50 (mM) | |
| 6 | 1 | reservoir | 1 (M) | ||
| 7 | 1 | reservoir | 0-0.6 (M) | ||
| 8 | 1 | reservoir | HEPES | 100 (mM) |
