1EIN
THE STRUCTURAL ORIGINS OF INTERFACIAL ACTIVATION IN THERMOMYCES (HUMICOLA) LANUGINOSA LIPASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 1996-06-01 |
Detector | RIGAKU RAXIS |
Spacegroup name | P 65 |
Unit cell lengths | 135.950, 135.950, 149.990 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.400 - 3.000 |
Rwork | 0.210 |
R-free | 0.24100 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.029 |
Data reduction software | ROTAVATA |
Data scaling software | CCP4 ((AGROVATA) |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.400 | 3.160 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.200 | 0.425 * |
Number of reflections | 31460 | |
<I/σ(I)> | 3 | |
Completeness [%] | 99.5 * | 98.2 * |
Redundancy | 5.2 | 2.1 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 8.1 | PEG 5000, magnesium chloride, c8e5 , pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 110K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 10 (mM) | |
3 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
4 | 1 | reservoir | 25 (mM) | ||
5 | 1 | reservoir | mPEG5000 | 17 (%(w/v)) |