1EGA

CRYSTAL STRUCTURE OF A WIDELY CONSERVED GTPASE ERA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-06-18
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	86.790, 67.560, 87.290
Unit cell angles	90.00, 115.82, 90.00

Refinement procedure

Resolution	8.000 - 2.400
Rwork	0.243
R-free	0.29800
Structure solution method	MIR
RMSD bond length	0.009
RMSD bond angle	1.550 *
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	X-PLOR (3.85)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.500
High resolution limit [Å]	2.400	2.400
Rmerge	0.056 *	0.490 *
Number of reflections	35696
<I/σ(I)>	31.8	3.9
Completeness [%]	99.7	99.1
Redundancy	6.4	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8		PROTEIN 8MG/ML TRIS BUFFER 0.1M, PH 8.0 LITHIUM SULFATE 0.8M SODIUM CHLORIDE 0.8M

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PROTEIN 8MG/ML
2	1	2	TRIS BUFFER 0.1M, PH 8.0
3	1	2	LITHIUM SULFATE 0.8M
4	1	2	SODIUM CHLORIDE 0.8M

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	reservoir	Tris-HCl	50 (mM)
3	1	reservoir		0.8 (M)