1EEU

M4L/Y(27D)D/Q89D/T94H mutant of LEN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	1999-07-06
Detector	CUSTOM-MADE
Spacegroup name	P 21 21 2
Unit cell lengths	62.900, 104.800, 42.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.600
R-factor	0.195 *
Rwork	0.194
R-free	0.22200
Structure solution method	Rigid body fitting
Starting model (for MR)	1EEQ WITH ATOMS BEYOND CB REMOVED FOR RESIDUES A 89 AND B 389
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.650
High resolution limit [Å]	1.600	1.600
Rmerge	0.061	0.263 *
Number of reflections	37705
<I/σ(I)>	43.6	7.3
Completeness [%]	99.7	99.6
Redundancy	18	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	298	20% PEG4K, 20% 2-Propanol, 0.1 M Sodium Citrate pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	20 (%)
2	1	reservoir	2-propanol	20 (%)
3	1	reservoir	sodium citrate	0.1 (M)	pH5.6
4	1	drop	protein	10 (mg/ml)