1EE9

CRYSTAL STRUCTURE OF THE NAD-DEPENDENT 5,10-METHYLENETETRAHYDROFOLATE DEHYDROGENASE FROM SACCHAROMYCES CEREVISIAE COMPLEXED WITH NAD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-04-09
Detector	RIGAKU RAXIS IV
Spacegroup name	P 41 21 2
Unit cell lengths	73.600, 73.600, 161.601
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.000
R-factor	0.25 *
Rwork	0.248
R-free	0.36000
Structure solution method	DIFFERENCE FOURIER
RMSD bond length	0.004
RMSD bond angle	1.216
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.077	0.578
Number of reflections	8474
<I/σ(I)>	11.3
Completeness [%]	81.3	73
Redundancy	3.3	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.2	4 *	Monzingo, A.F., (1996) Proteins: Struct., Funct., Genet., 26, 481. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	7 (%)
2	1	reservoir	Tris-HCl	50 (mM)