1EE4

CRYSTAL STRUCTURE OF YEAST KARYOPHERIN (IMPORTIN) ALPHA IN A COMPLEX WITH A C-MYC NLS PEPTIDE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Spacegroup name	P 1 21 1
Unit cell lengths	42.680, 85.850, 117.500
Unit cell angles	90.00, 93.42, 90.00

Refinement procedure

Resolution	30.000 - 2.100
R-factor	0.246 *
Rwork	0.246
R-free	0.27700
RMSD bond length	0.016
RMSD bond angle	1.740
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	30.000
High resolution limit [Å]	2.100	2.100
Rmerge	0.085	0.213 *
Total number of observations	152954 *
Number of reflections	47949 *
<I/σ(I)>	15.8
Completeness [%]	96.9	96.9 *
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	4 *	pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	Tris-HCl	100 (mM)
3	1	reservoir	sodium acetate	200 (mM)
4	1	reservoir	PEG4000	30 (%(w/v))