1EDZ

STRUCTURE OF THE NAD-DEPENDENT 5,10-METHYLENETETRAHYDROFOLATE DEHYDROGENASE FROM SACCHAROMYCES CEREVISIAE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-03-16
Detector	RIGAKU RAXIS IV
Spacegroup name	P 41 21 2
Unit cell lengths	73.980, 73.980, 161.685
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.800
R-factor	0.26 *
Rwork	0.260
R-free	0.35000 *
RMSD bond length	0.005
RMSD bond angle	1.217
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.092	0.263
Number of reflections	10691 *
<I/σ(I)>	12
Completeness [%]	97.8	96.3
Redundancy	4.8	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.2	4 *	Monzingo, A.F., (1996) Proteins: Struct., Funct., Genet., 26, 481. *
1	Vapor diffusion, hanging drop *	8.2	4 *	Monzingo, A.F., (1996) Proteins: Struct., Funct., Genet., 26, 481. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	7 (%)
2	1	reservoir	Tris-HCl	50 (mM)