1EBV
OVINE PGHS-1 COMPLEXED WITH SALICYL HYDROXAMIC ACID
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-04-02 |
| Detector | MARRESEARCH |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 98.870, 207.460, 221.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.500 - 3.200 |
| R-factor | 0.218 * |
| Rwork | 0.218 |
| R-free | 0.24800 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 * | 3.400 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.113 | 0.289 * |
| Total number of observations | 124009 * | |
| Number of reflections | 35021 * | |
| <I/σ(I)> | 13 | |
| Completeness [%] | 82.0 | 75 * |
| Redundancy | 4 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 291 | PEG4000, sodium phosphate, octyl glucoside, sodium chloride, diethyldithiocarbamate, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 6.5 (%) | |
| 2 | 1 | reservoir | sodium phosphate | 65 (mM) | pH6.7 |
| 3 | 1 | reservoir | 410 (mM) | ||
| 4 | 1 | drop | enzyme | 12 (mg/ml) | |
| 5 | 1 | drop | sodium phosphate | 20 (mM) | pH6.7 |
| 6 | 1 | drop | 100 (mM) | ||
| 7 | 1 | drop | beta-octylglucoside | 0.6 (%(w/w)) | |
| 8 | 1 | drop | diethyldithiocarbamate | 0.100 (mM) |
