1EBF
HOMOSERINE DEHYDROGENASE FROM S. CEREVISIAE COMPLEX WITH NAD+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 218 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-01-16 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 80.396, 80.396, 250.165 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.960 - 2.300 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.26900 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 23.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.077 | 0.490 |
| Number of reflections | 188533 | |
| <I/σ(I)> | 16.7 | |
| Completeness [%] | 98.4 | 94.8 |
| Redundancy | 5.4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 * | 295 | Ammonium sulphate, Calcium chloride, NAD+, Sodium Acetate , pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | or 10 mM HEPES |
| 3 | 1 | drop | 20 (mM) | ||
| 4 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
| 5 | 1 | reservoir | Tris-HCl | 0.1 (M) |
