1EAZ
Crystal structure of the phosphoinositol (3,4)-bisphosphate binding PH domain of TAPP1 from human.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-02-25 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 65.017, 102.796, 41.471 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.400 |
| R-factor | 0.171 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fb8 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.980 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.058 | 0.115 |
| Number of reflections | 27661 | |
| <I/σ(I)> | 21.7 | 4.2 |
| Completeness [%] | 98.9 | 97.7 |
| Redundancy | 4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.6 | 20 * | 0.085M SODIUM CITRATE, 25.5% PEG 4000, 15% GLYCEROL, 0.17M AMMONIUM ACETATE, pH 5.60 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 14.5 (mg/ml) | |
| 2 | 1 | reservoir | sodium citrate | 0.085 (M) | pH5.6 |
| 3 | 1 | reservoir | PEG4000 | 25.5 (%(w/v)) | |
| 4 | 1 | reservoir | glycerol | 15 (%(v/v)) | |
| 5 | 1 | reservoir | ammonium acetate | 0.17 (M) |
