1E6C
K15M MUTANT OF SHIKIMATE KINASE FROM ERWINIA CHRYSANTHEMI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1998-02-15 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.860, 106.940, 42.750 |
Unit cell angles | 90.00, 119.96, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.800 |
R-factor | 0.188 * |
Rwork | 0.188 |
R-free | 0.22700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1shk |
RMSD bond length | 0.015 |
RMSD bond angle | 0.038 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.400 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.078 | 0.348 |
Total number of observations | 164162 * | |
Number of reflections | 27407 | |
<I/σ(I)> | 15 | 1.9 |
Completeness [%] | 93.0 | 69 * |
Redundancy | 5.3 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7.5 * | 10% PEG8000, 100MM TRIS/HCL BUFFER PH 8.0, 2.5MM ADP, 2.5MM SHIKIMATE, 10MM MGCL2 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8-10 (mg/ml) | |
2 | 1 | reservoir | PEG8000 | 10 (%) | |
3 | 1 | reservoir | Tris-HCl | 100 (mM) |