1E50
AML1/CBFbeta complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 1999-02-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 103.260, 79.380, 130.100 |
| Unit cell angles | 90.00, 101.39, 90.00 |
Refinement procedure
| Resolution | 23.820 - 2.600 |
| R-factor | 0.268 |
| Rwork | 0.268 |
| R-free | 0.28700 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 24.800 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.085 | 0.280 * |
| Total number of observations | 259515 * | |
| Number of reflections | 63354 | |
| <I/σ(I)> | 5.6 | 1.3 |
| Completeness [%] | 99.3 | 99.2 * |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 21 * | used macroseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | imidazole | 100 (mM) | |
| 2 | 1 | reservoir | PEG8000 | 6 (%) | |
| 3 | 1 | reservoir | sucrose | 5 (%) | |
| 4 | 1 | reservoir | dithiothreitol | 5 (mM) | |
| 5 | 1 | drop | protein | 12 (mg/ml) |
