1E3U
MAD structure of OXA10 class D beta-lactamase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-04-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.95375, 1.0376 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.530, 82.940, 101.420 |
| Unit cell angles | 90.00, 95.03, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.660 |
| R-factor | 0.1806 * |
| Rwork | 0.181 |
| R-free | 0.20966 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.016 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.100 | 1.750 |
| High resolution limit [Å] | 1.653 | 1.650 |
| Rmerge | 0.064 * | 0.322 * |
| Total number of observations | 598857 * | |
| Number of reflections | 127968 | |
| <I/σ(I)> | 8.1 | 2.1 |
| Completeness [%] | 98.9 | 98.9 * |
| Redundancy | 4.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.8 * | 4 * | AMMONIUMS SULFATE 2.0 M, TRIS HCL 100 MM, PH 8.2-8.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | sodium potassium phosphate | 20 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 2.2 (M) | |
| 4 | 1 | reservoir | Tris-HCl | 0.1 (M) |
