1E2X
FadR, fatty acid responsive transcription factor from E. coli
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-04-15 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9535, 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 59.711, 101.973, 87.513 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.480 - 2.000 |
R-factor | 0.2 |
Rwork | 0.200 |
R-free | 0.23700 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 19.300 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS (1.0) |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.070 | 0.448 |
Number of reflections | 18125 | |
<I/σ(I)> | 9.7 | 2.6 |
Completeness [%] | 95.2 | 73.8 * |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8 * | LI2SO4/(NH4)2SO4, TRIS PH 9 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 4.6 (mg/ml) | |
2 | 1 | drop | 50 (mM) | ||
3 | 1 | drop | glycerol | 10 (%) | |
4 | 1 | reservoir | ammonium sulfate | 2.3 (M) | |
5 | 1 | reservoir | 0.75 (M) | ||
6 | 1 | reservoir | Tris-HCl | 100 (mM) |