1E2A
ENZYME IIA FROM THE LACTOSE SPECIFIC PTS FROM LACTOCOCCUS LACTIS
Experimental procedure
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X31 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1995-10-01 |
Detector | MARRESEARCH |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 90.900, 90.900, 82.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.300 |
R-factor | 0.19 |
Rwork | 0.190 |
R-free | 0.24000 |
Structure solution method | SINGLE ISOMORPHOUS REPLACEMENT AND ANOMALOUS SCATTERING (SIRAS) |
RMSD bond length | 0.005 |
RMSD bond angle | 16.940 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.086 * | 0.363 * |
Number of reflections | 16632 | |
<I/σ(I)> | 18.3 | 4.9 |
Completeness [%] | 97.7 | 98.9 |
Redundancy | 6.1 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 25 * | CRYSTALS WERE OBTAINED BY THE SITTING-DROP VAPOR-DIFFUSION TECHNIQUE AT ROOM TEMPERATURE USING A SMALL INCREASE IN THE PHOSPHATE BUFFER. THE PROTEIN SOLUTION WAS MIXED WITH 0.15M NA/K PHOSPHATE BUFFER, PH 6.4. THE LARGEST CRYSTALS APPEARED IN CIRCA A WEEK., vapor diffusion - sitting drop |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | sodium potassium phosphate | 150 (mM) | |
3 | 1 | reservoir | sodium potassium phosphate | 400 (mM) |