1E21

Ribonuclease 1 des1-7 Crystal Structure at 1.9A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2000-02-15
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	32.691, 42.733, 79.894
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.900
R-factor	0.1959
Rwork	0.196
R-free	0.25110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7rsa
RMSD bond length	0.007
RMSD bond angle	1.610
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.135	0.364 *
Total number of observations	27390 *
Number of reflections	8618
<I/σ(I)>	4.4	2
Completeness [%]	92.9	87.2
Redundancy	3.2	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5		7% PEG 8K, 0.1M TRIS-HCL PH 8.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	PEG8000	7 (%(v/v))
3	1	reservoir	Tris-HCl	0.1 (M)