1E1X

HUMAN CYCLIN DEPENDENT KINASE 2 COMPLEXED WITH THE INHIBITOR NU6027

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	CCD
Spacegroup name	P 21 21 21
Unit cell lengths	52.650, 69.900, 71.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 1.850
R-factor	0.213 *
Rwork	0.213
R-free	0.28100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hck
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CCP4
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.930
High resolution limit [Å]	1.850	1.850
Rmerge	0.057	0.372
Total number of observations	62274 *
Number of reflections	22399
<I/σ(I)>	16.3	2.27
Completeness [%]	96.4	97.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	other *	7.4		Lawrie, A.M., (1997) Nature Struct. Biol., 4, 796. *