1E12
Halorhodopsin, a light-driven chloride pump
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-08-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 67.300, 67.300, 209.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 1.800 |
| R-factor | 0.237 |
| Rwork | 0.237 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1brr |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.775 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.051 | 0.313 |
| Number of reflections | 26590 | |
| <I/σ(I)> | 26.5 | 2.8 |
| Completeness [%] | 98.2 | 92.3 |
| Redundancy | 6.6 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 7 | CRYSTALLIZED IN A CUBIC LIPID PHASE MADE OF 58-62 W/V % 1-MONOOLEIN, 4 M KCL, 3.3-4.0 MG/ML HR AND 50 MM TRIS/HCL, PH 7. |
