1DZA
3-D structure of a HP-RNase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7A |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 1998-05-15 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 27.850, 67.420, 114.140 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.900 - 1.650 |
R-factor | 0.177 |
Rwork | 0.185 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7rsa |
RMSD bond length | 0.009 |
RMSD bond angle | 0.026 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.900 | 1.690 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.133 | 0.337 |
Total number of observations | 80829 * | |
Number of reflections | 25881 | |
<I/σ(I)> | 3.3 | 2.9 |
Completeness [%] | 96.6 | 63.2 |
Redundancy | 3.1 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.5 | pH 8.50 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | 10 (mg/ml) | ||
2 | 1 | reservoir | PEG4000 | 30-35 (%(v/v)) | |
3 | 1 | reservoir | Tris-HCl | 0.1 (M) |