1DYT
X-ray crystal structure of ECP (RNase 3) at 1.75 A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-03-15 |
Detector | MARRESEARCH |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 62.163, 62.163, 174.590 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.750 |
R-factor | 0.224 |
Rwork | 0.224 |
R-free | 0.27100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | EOSINOPHIL DERIVED PROTEIN |
RMSD bond length | 0.013 |
RMSD bond angle | 1.752 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.000 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.033 | 0.030 |
Total number of observations | 58607 * | |
Number of reflections | 35401 | |
<I/σ(I)> | 16.3 | 1.75 |
Completeness [%] | 99.5 | 99.9 |
Redundancy | 1.9 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.2 | 8% JEFFAMINE M-600, 0.1M SODIUM CITRATE PH 5.2, 0.01M IRON CHLORIDE |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 11 (mg/ml) | |
2 | 1 | reservoir | Jeffamine M-600 | 8 (%(v/v)) | |
3 | 1 | reservoir | sodium citrate | 0.1 (M) | |
4 | 1 | reservoir | 0.01 (M) |