1DY2

Murine collagen alpha1(XV), endostatin domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1999-03-15
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	33.550, 57.700, 71.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.2
Rwork	0.200
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1koe
RMSD bond length	0.006
RMSD bond angle	1.500
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.120
High resolution limit [Å]	2.000	2.000
Rmerge	0.060 *	0.152
Number of reflections	9225
Completeness [%]	97.1	97.2
Redundancy	4.7	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *		pH 6.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris-HCl	0.01 (M)
3	1	reservoir	PEG8000	20-30 (%(w/v))
4	1	reservoir	ammonium sulfate	0.3 (M)
5	1	reservoir	sodium cacodylate	0.1 (M)