1DY1
Murine endostatin, crystal form III
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ELLIOTT GX-21 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1998-09-15 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.210, 52.230, 96.450 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.200 |
R-factor | 0.195 |
Rwork | 0.195 |
R-free | 0.27000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1koe |
RMSD bond length | 0.010 |
RMSD bond angle | 1.700 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.106 | 0.289 |
Total number of observations | 72598 * | |
Number of reflections | 9988 | |
Completeness [%] | 99.3 | 97.9 * |
Redundancy | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.8 * | pH 8.50 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | drop | sodium MOPS | ||
3 | 1 | reservoir | PEG4000 | 10 (%(w/v)) | |
4 | 1 | reservoir | 300 (mM) | ||
5 | 1 | reservoir | 10 (mM) | ||
6 | 1 | reservoir | Tris-HCl | 100 (mM) |