1DXQ
CRYSTAL STRUCTURE OF MOUSE NAD[P]H-QUINONE OXIDOREDUCTASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | R-AXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.960, 171.620, 67.140 |
| Unit cell angles | 90.00, 112.30, 90.00 |
Refinement procedure
| Resolution | 42.980 - 2.800 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qrd |
| RMSD bond length | 0.010 |
| RMSD bond angle | 21.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 42.980 |
| High resolution limit [Å] | 2.800 |
| Rmerge | 0.100 * |
| Number of reflections | 18847 |
| Completeness [%] | 80.0 * |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 25 * | pH 8.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-15 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 25 (mM) | |
| 3 | 1 | drop | FAD | 0.005 (mM) | |
| 4 | 1 | reservoir | PEG3350 | 30 (%) | |
| 5 | 1 | reservoir | sodium acetate | 200 (mM) | |
| 6 | 1 | reservoir | sodium tricine | 100 (mg/ml) | |
| 7 | 1 | reservoir | FAD | 0.012-0.024 (mM) |
