1DWX
MURINE INDUCIBLE NITRIC OXIDE SYNTHASE OXYGENASE DIMER N-hydroxyarginine and tetrahydrobiopterin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-02-15 |
Detector | MARRESEARCH |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 212.980, 212.980, 114.200 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.600 |
R-factor | 0.238 |
Rwork | 0.238 |
R-free | 0.27800 |
Structure solution method | OTHER |
RMSD bond length | 0.008 * |
RMSD bond angle | 1.400 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.8) |
Refinement software | X-PLOR (3.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.720 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.057 * | 0.328 * |
Total number of observations | 153562 * | |
Number of reflections | 46622 | |
<I/σ(I)> | 18.6 | 2.8 |
Completeness [%] | 97.8 | 88.1 |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.6 * | 4 * | pH 6.00 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 17 (mg/ml) | |
10 | 1 | reservoir | 18-21 (%) | or ammonium sulfate | |
2 | 1 | drop | HEPPS | 40 (mM) | |
3 | 1 | drop | glycerol | 10 (%) | |
4 | 1 | drop | dithiothreitol | 1 (mM) | |
5 | 1 | drop | NHA | 4 (mM) | |
6 | 1 | drop | 2 (mM) | ||
7 | 1 | drop | 4-amino-H4B | 2 (mM) | |
8 | 1 | reservoir | MES | 50 (mM) | |
9 | 1 | reservoir | beta-octylglucoside | 50 (mM) |