1DVM

ACTIVE FORM OF HUMAN PAI-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	1998-10-22
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 1
Unit cell lengths	64.764, 74.674, 103.445
Unit cell angles	85.19, 86.17, 64.34

Refinement procedure

Resolution	30.000 - 2.400
R-factor	0.175 *
Rwork	0.218
R-free	0.27100 *
RMSD bond length	0.009
RMSD bond angle	1.500
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (98.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.470
High resolution limit [Å]	2.400	2.400
Rmerge	0.093	0.221
Total number of observations	211004 *
Number of reflections	67997 *
<I/σ(I)>	7.6
Completeness [%]	88.8	78.4
Redundancy	3.1 *	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.1	293	NaCl, MES, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 293.K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	11 (mg/ml)
2	1	drop	MES	20 (mM)
3	1	drop		1 (M)
4	1	reservoir		1.7-2.0 (M)