1DTG

HUMAN TRANSFERRIN N-LOBE MUTANT H249E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1996-10-15
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 41 21 2
Unit cell lengths	72.660, 72.660, 152.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.400
Rwork	0.198
R-free	0.29400
RMSD bond length	0.009
RMSD bond angle	1.680
Data reduction software	DENZO
Data scaling software	CCP4 ((AGROVATA)
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.500
High resolution limit [Å]	2.400	2.400
Rmerge	0.079	0.239
Number of reflections	15387 *
<I/σ(I)>	11.6
Completeness [%]	91.8	96.1
Redundancy	4.7	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.1	4 *	50 mM cacodylate 40% ethanol, pH 6.1, VAPOR DIFFUSION, SITTING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	potassium cacodylate	50 (mM)
2	1	drop	ethanol	40 (%(v/v))
3	1	reservoir	ethanol	40 (%(v/v))
4	1	reservoir	potassium cacodylate	50 (mM)