1DSZ
STRUCTURE OF THE RXR/RAR DNA-BINDING DOMAIN HETERODIMER IN COMPLEX WITH THE RETINOIC ACID RESPONSE ELEMENT DR1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 113 |
Detector technology | AREA DETECTOR |
Collection date | 1995-05-11 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 80.660, 33.900, 101.860 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.700 |
R-factor | 0.201 |
Rwork | 0.198 |
R-free | 0.26700 |
RMSD bond length | 0.009 |
RMSD bond angle | 2.100 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.071 | 0.170 |
Number of reflections | 29834 | |
<I/σ(I)> | 19.9 | |
Completeness [%] | 94.8 | 98 |
Redundancy | 4.1 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 8 * | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Tris buffer | ||
2 | 1 | 1 | NH4Cl | ||
3 | 1 | 1 | MgCl2 | ||
4 | 1 | 1 | PEG 3350 | ||
5 | 1 | 2 | PEG 3350 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 0.90 (mM) | |
2 | 1 | drop | DNA | 0.45 (mM) | |
3 | 1 | drop | Tris-HCl | 25 (mM) | |
4 | 1 | reservoir | PEG3350 | 18-23 (%) | |
5 | 1 | reservoir | Tris-HCl | 25 (mM) | |
6 | 1 | reservoir | 5 (mM) | ||
7 | 1 | reservoir | 0.4 (M) |