1DSY
C2 DOMAIN FROM PROTEIN KINASE C (ALPHA) COMPLEXED WITH CA2+ AND PHOSPHATIDYLSERINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-02-25 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 58.800, 58.800, 91.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.000 - 2.600 |
| Rwork | 0.195 |
| R-free | 0.23300 * |
| RMSD bond length | 0.015 * |
| RMSD bond angle | 1.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.092 | 0.281 |
| Total number of observations | 17072 * | |
| Number of reflections | 5610 | |
| <I/σ(I)> | 9.4 | |
| Completeness [%] | 94.3 | 95.1 |
| Redundancy | 2.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 20 * | PEG8K, POTASSIUM PHOSPHATE, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4-8 (mg/ml) | |
| 2 | 1 | drop | calcium chloride | 25 (mM) | |
| 3 | 1 | drop | DCPS | 2 (mM) | |
| 4 | 1 | drop | 25 (mM) | ||
| 5 | 1 | reservoir | PEG8000 | 20 (%) | |
| 6 | 1 | reservoir | potassium phosphate | 50 (mM) |
