1DST
MUTANT OF FACTOR D WITH ENHANCED CATALYTIC ACTIVITY
Experimental procedure
| Temperature [K] | 295 |
| Detector technology | AREA DETECTOR |
| Detector | SIEMENS |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 45.380, 45.380, 175.210 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 7.500 - 2.000 |
| R-factor | 0.198 * |
| R-free | 0.21300 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 0.035 |
| Data reduction software | XENGEN |
| Data scaling software | XENGEN |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.076 |
| Total number of observations | 59734 * |
| Number of reflections | 13899 * |
| Completeness [%] | 94.0 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.8 | 22 * | pH 6.8 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MES | 50 (mM) | |
| 2 | 1 | reservoir | PEG6000 |
