1DST

MUTANT OF FACTOR D WITH ENHANCED CATALYTIC ACTIVITY

Experimental procedure

Temperature [K]	295
Detector technology	AREA DETECTOR
Detector	SIEMENS
Spacegroup name	P 31 2 1
Unit cell lengths	45.380, 45.380, 175.210
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	7.500 - 2.000
R-factor	0.198 *
R-free	0.21300
RMSD bond length	0.020
RMSD bond angle	0.035
Data reduction software	XENGEN
Data scaling software	XENGEN
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.000
Rmerge	0.076
Total number of observations	59734 *
Number of reflections	13899 *
Completeness [%]	94.0
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.8	22 *	pH 6.8

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	MES	50 (mM)
2	1	reservoir	PEG6000