1DRV

ESCHERICHIA COLI DHPR/ACNADH COMPLEX

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	290
Detector technology	AREA DETECTOR
Collection date	1995-09-26
Detector	SIEMENS
Spacegroup name	I 2 2 2
Unit cell lengths	81.600, 84.600, 91.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.200
R-factor	0.191 *
Rwork	0.191
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	DHPR-NADPH COMPLEX
RMSD bond length	0.018
RMSD bond angle	20.900 *
Data reduction software	SADIE
Data scaling software	SAINT
Phasing software	AMoRE
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.094 *
Number of reflections	14402
<I/σ(I)>	7.1	2.1
Completeness [%]	87.3 *	78.1
Redundancy	3.5	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5		Scapin, G., (1995) Biochemistry, 34, 3502. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium salfate	2.2 (M)
2	1	reservoir	HEPES	100 (mM)	pH7.5
3	1	drop	protein	18 (mg/ml)
4	1	drop	ammonium salfate	2.2 (M)
5	1	drop	HEPES	100 (mM)
6	1	drop	NADPH	1.5-2.0 (M)