1DRB
CRYSTAL STRUCTURE OF UNLIGANDED ESCHERICHIA COLI DIHYDROFOLATE REDUCTASE. LIGAND-INDUCED CONFORMATIONAL CHANGES AND COOPERATIVITY IN BINDING
Experimental procedure
| Spacegroup name | P 61 |
| Unit cell lengths | 92.840, 92.840, 74.240 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 5.000 - 1.960 |
| R-factor | 0.149 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 0.025 |
| Refinement software | PROLSQ |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.4 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 24 (mg/ml) | unliganded |
| 2 | 1 | drop | sodium/potassium phthalate | 50 (mM) | |
| 3 | 1 | drop | PEG6000 | 50 (%(w/w)) | |
| 4 | 1 | reservoir | PEG6000 | 25 (%) |
