1DQT

THE CRYSTAL STRUCTURE OF MURINE CTLA4 (CD152)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9B
Synchrotron site	NSLS
Beamline	X9B
Temperature [K]	100
Detector technology	AREA DETECTOR
Collection date	1999-06-28
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	112.850, 112.850, 102.504
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.2217
Rwork	0.211
R-free	0.25430
RMSD bond length	0.009
RMSD bond angle	27.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.062 *	0.363 *
Total number of observations	301486 *
Number of reflections	44587
<I/σ(I)>	36.5
Completeness [%]	99.4 *	95.7
Redundancy	13.7	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	18 *	18% PEG 6000, 2.0 M NaCl, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 18.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG6000	20 (%)
3	1	reservoir		2.0 (M)