1DP0

E. COLI BETA-GALACTOSIDASE AT 1.7 ANGSTROM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	95
Detector technology	CCD
Collection date	1998-03-12
Detector	ADSC QUANTUM 4
Spacegroup name	P 21 21 21
Unit cell lengths	149.600, 168.380, 200.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 1.700
R-factor	0.157 *
Rwork	0.157
R-free	0.21100
Structure solution method	MOLECULAR REPLACEMEN
RMSD bond length	0.018
RMSD bond angle	2.916
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.060	0.346
Total number of observations	2201152 *
Number of reflections	542319
<I/σ(I)>	17.5	2.9
Completeness [%]	98.6	97 *
Redundancy	4.2	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.5	288	used seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)	pure tetramer
2	1	reservoir	PEG8000	10 (%)
3	1	reservoir	Bis-Tris	100 (mM)
4	1	reservoir		200 (mM)
5	1	reservoir		100 (mM)
6	1	reservoir	dithiothreitol	10 (mM)